ABSTRACT
Objective To observe the effect of the Mongolian medicine on the apoptosis after spinal cord injury. Methods A total of 12 rats were divided into the sham group, the model group, and the like-3 treatment groups (Naru-3 group), 4 in each group. A rat model of spinal cord injury (SCI) was established by modified Allen's method. After 72 h, we sacrificed the rats, separated spinal cord cells, which were filled with 5×105 shop in every hole, training for subsequent testing after 24 h. The relative expression of caspase-3 was detected by RT-QPCR and western blot, and apoptosis was analyzed by flow cytometry. Results Compared to the model group, the expression of caspase-3 mRNA (2.177 ± 0.084 vs. 3.287 ± 0.098) and caspase-3 protine (1.063 ± 0.157 vs. 2.180 ± 0.147) in the Naru-3 group significantly decreased (P<0.05). The apoptosis rate (11.400% ± 0.953% vs. 14.260% ± 1.105%) of the spinal cord in the Naru-3 group significantly decreased (P<0.05). Conclusions The Naru-3 solution can reduce the rate of spinal cord cell apoptosis after spinal cord injury, its mechanism may be related to the suppressed caspase-3 protein expression.
ABSTRACT
Objective@#To observe the effect of the Mongolian medicine on the apoptosis after spinal cord injury.@*Methods@#A total of 12 rats were divided into the sham group, the model group, and the like-3 treatment groups (Naru-3 group), 4 in each group. A rat model of spinal cord injury (SCI) was established by modified Allen's method. After 72 h, we sacrificed the rats, separated spinal cord cells, which were filled with 5×105 shop in every hole, training for subsequent testing after 24 h. The relative expression of caspase-3 was detected by RT-QPCR and western blot, and apoptosis was analyzed by flow cytometry.@*Results@#Compared to the model group, the expression of caspase-3 mRNA (2.177 ± 0.084 vs. 3.287 ± 0.098) and caspase-3 protine (1.063 ± 0.157 vs. 2.180 ± 0.147) in the Naru-3 group significantly decreased (P<0.05). The apoptosis rate (11.400% ± 0.953% vs. 14.260% ± 1.105%) of the spinal cord in the Naru-3 group significantly decreased (P<0.05).@*Conclusions@#The Naru-3 solution can reduce the rate of spinal cord cell apoptosis after spinal cord injury, its mechanism may be related to the suppressed caspase-3 protein expression.